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^: 2660 2012 3
^: .. ( - . ).; ..; ..
^: Oriza L. ()
^: .-... , 2012; N 3. - . 12-18
^: 2012
^: . ..-.:.17-18
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^: While introducing gene-modified crops in agricultural practice a problem of vertical gene transfer from gene-modified to conventional varieties as a consequence of pollen application, intercross and transgenic hybrid seed production. The investigations by evaluating the frequency of vertical transfer were conducted for 2 years. As pollen donors the transgenic homozygous line of spring wheat variety Andros comprising heterological sequences of gene bar imparting resistance to phosphinotricin-based herbicides and green fluorescent protein gene gfp. The transgenic variety was sown inside a circle having a diameter of 1 m, and the non-transgenic analog - in the distance of 1, 2 and 3 m from the outside perimeter. The relationship of the transgenic transfer frequency and prevailing wind direction has been discovered. In the field sector located in this direction 81 and 84% of the total amount of detected transgenic seeds have been found. When removing non-transgenic plants from transgenic ones 1 m the transfer frequency was 0.134%, 2 m - 0.035%, 3 m - 0.002%. In general, the frequency of a transgenic vertical drift to seeds of non-transgenic plants in the field areas varied from 0.000 to 0.797%. The transgenic status of hybrid seeds was confirmed by the resistance to the applied herbicides, tissue fluorescence, and the presence of transgenic sequences in the plant genome (the data of PCR and reverse transcription PCR, as well as typical inheritance of transgenes in the following seed generation aref1

^TRN: 1281407
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