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^: 1659 2012 5
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^: ...-.., 2012; N 5. - . 29-37
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^: - (). 26 , .. , . : (), SDS-PAGE , -, , BLAST-. 23 , () . , 15 23 . "" , , , . 2 (1670 197/2-1-2trs) . 2 1152 .. 1670 1352 .. 197/2-1-2trs. BLAST- Thynopyrum intermedium Th. elongatum. , 1670, Th. intermedium, , 197/2-1-2trs, , Lophopyrum elongatum ( Th. longatum). , .

aref2 The aim of the paper is to study the genes of wheatgrass storage proteins in wheat-wheatgrass hybrids (WWH). The study was carried out using seeds of 26 lines of WWH developed in the Department of Distance Hybridization of Tsitsin Main Botanical (Russian Academy of Sciences) using medium and ponticum wheatgrass. The analysis was performed using polymerase chain reaction (PCR), SDS-PAGE storage protein electrophoresis, PCR-product cloning, sequencing, and BLAST-analysis. 23 WWH with the genes of wheatgrass high-molecular (HMW) glutenins have been detected. The expression of these genes at least in 15 of 23 lines has been shown using protein electrophoresis. It can result from either the "silence" of the genes or the similarity or identity of the expressed wheat and wheatgrass protein subunits in molecular weight, so, they cannot be distinguished with one-dimensional electrophoresis. Wheatgrass storage protein genes of two wheat-wheatgrass lines (1670 and 197/2-1-2trs) were cloned and sequenced. As a result, two different nuclei sequences have been described: 1152 b.p. for 1670 and 1352 b.p. for 197/2-1-2trs. BLAST-analysis of the sequences showed the relation of them to HMW glutenin genes of Thinopyrum intermedium and Th. elongatum. The gene revealed in 1670 is clasterized with the HMW glutenin gene of Th. intermedium, and that of 197/2-1-2trs with Lophopyrum elongatum (syn. Th. elongatum). The found lines are interesting as an object to study the influence of wheatgrass HMW glutenins on gluten, storage proteins compound and bread-making quality.

^TRN: 1291793
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