-
()
-


@Mail.ru .
[ ]

^: *http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1445-6664 /issues (http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1445-6664/ issues)
^: Keith B.K.; Kalinina E.B.; Dyer W.E.
^: Differentially expressed genes in dicamba-resistant and dicamba-susceptible biotypes [ (Kochia scoparia). ]
^: Weed Biology & Management, 2011; Vol.11,N 4. - P. 224-234
^: 2011
+

^: In an ongoing effort to investigate the mechanism of auxinic herbicide resistance in Kochia scoparia (kochia), polymerase chain reaction-based cDNA suppression subtractive hybridization was used to identify genes that are differentially expressed between dicamba-resistant (HRd) and dicamba-susceptible (S1) kochia biotypes in response to herbicide treatment. Both the HRd and S1 adaptor-ligated cDNAs were used in separate hybridizations in order to generate biotype-specific clones. A total of 710 cDNAs, representing putative differentially expressed mRNAs, were isolated and subjected to further screening. The false-positive cDNAs were removed by conducting two colony hybridizations and at least one Northern hybridization. Differential or biotype-specific expression was confirmed for six clones each from the HRd and S1 plants. The S1-related genes were constitutively expressed at higher levels than in the HRd plants, but none had significant sequence similarity to known genes. Among the HRd-related genes, HRd-88 had 42% amino acid sequence identity to a conserved domain within thiol peptidases, which might be involved in auxin-regulated gene expression. The constitutively expressed and inducible (by the dicamba treatment) HRd-39 had 40% identity and 60% similarity to a domain from the Fe(II)/α-ketoglutarate-dependent hydroxylase superfamily. The HRd-39 gene product had the characteristics of an enzyme that is able to detoxify dicamba via oxidative hydroxylation and thus its overexpression might confer the dicamba resistance phenotype. aref1

^TRN: 1375536
^:
^:
+



        

1998-2017 ©