^АВТ: Cabanas D.; Watanabe S.; Higashi C.H.V.;
Bressan A.; Higashi
; C.H.V.; Bressan
^ЗГЛ: Dissecting the Mode of Maize Chlorotic Mottle Virus
Transmission (Tombusviridae: Machlomovirus) by Frankliniella
williamsi (Thysanoptera: Thripidae) [Исследование
механизма передачи вируса хлоротической пятнистости кукурузы
трипсом Frankliniella williamsi (Thysanoptera: Thripidae) на
Гавайских о-вах. (США)]
^ВЫХ: Journal of Economic Entomology, 2013; Vol.106,N 1. - P.
^РЕФ: Maize chlorotic mottle virus (MCMV) (Tombusviridae: Machlomovirus) has been recorded in Hawaii (Kauai Island) since the early 1990s and has since become one of the most widespread corn viruses in the Hawaiian Islands. In the United States Mainland, MCMV has been reported to be transmitted by six different species of chrysomelid beetles, including the western corn rootworm, Diabrotica virgifera virgifera LeConte. However, none of these beetle species have been reported in Hawaii where the corn thrips, Frankliniella williamsi Hood (Thysanoptera: Thripidae) has been identified to be the main vector. In this study, we developed leaf disk transmission assays and real time reverse transcription-polymerase chain reaction to examine the mode of MCMV transmission by the corn thrips. We showed that thrips transmitted the virus with no evidence for latent periods. Both larvae and adults transmitted the virus for up to 6 d after acquisition, with decreasing rates of transmission as time progressed. There was no evidence that adult thrips that acquired the virus as larvae were competent vectors. Real time reverse-transcription polomerase chain reaction assays showed that viral load was depleted from the vector's body after thrips had access to healthy plant tissue. Depletion of viral load was also observed when thrips matured from larvae to adults. Thrips were able to transmit MCMV after acquisition and inoculation access periods of 3 h. However, transmission efficiency increased with longer acquisition and inoculation access periods. Taken altogether our data suggests that corn thrips transmit MCMV in a semipersistent manner. To our knowledge, this is the first work reporting evidence of a plant virus transmitted semipersistently by thrips.
^ВИД: Статья из книги
^РУБ: 68_37_31_29_15; 68_37_29_29_17
^УДК: 633.15:632.38; 633.15:632.731
^ТЕР: КУКУРУЗА. ZEA MAYS. ВИРУСНЫЕ БОЛЕЗНИ РАСТЕНИЙ [ВИРОЗ
РАСТЕНИЙ]. ВИРУС ХЛОРОТИЧЕСКОЙ ПЯТНИСТОСТИ КУКУРУЗЫ
(MAIZE CHLOROTIC MOTTLE VIRUS). ПУТИ ПЕРЕДАЧИ ВОЗБУДИТЕЛЯ.
ПЕРЕНОСЧИКИ (Vectors). FRANKLINIELLA. ГАВАЙСКИЕ ОСТРОВА
^РТЗ: MACHLOMOVIRUS [МАХЛОМОВИРУСЫ]. POACEAE [GRAMINEAE;
ЗЛАКИ; МЯТЛИКОВЫЕ]. THRIPIDAE. THYSANOPTERA
[БАХРОМЧАТОКРЫЛЫЕ; ТРИПСЫ]. TOMBUSVIRIDAE. ZEA
[EUCHLAENA]. АМЕРИКА (Americas). БОЛЕЗНИ РАСТЕНИЙ (Plant
diseases). ВИРУСЫ РАСТЕНИЙ (Plant viruses)
[ФИТОПАТОГЕННЫЕ ВИРУСЫ]. ЗАРАЖЕНИЕ (Infection). ЗЕРНОВЫЕ
КУЛЬТУРЫ (Grain crops). ИНФЕКЦИОННЫЕ БОЛЕЗНИ РАСТЕНИЙ.
НАСЕКОМЫЕ (Insects) [INSECTA]. ОКЕАНИЯ (Oceania).
ОРГАНИЗМЫ. С-Х КУЛЬТУРЫ. СЕВЕРНАЯ АМЕРИКА (North America).
СТРАНЫ АТЭС. СТРАНЫ МИРА. США (USA). ФИТОПАТОГЕНЫ.
ЧЛЕНИСТОНОГИЕ (Arthropods) [ARTHROPODA].
^КЛС: Frankliniella williamsi.