^ШХР: *http://esa.publisher.ingentaconnect.com/content/esa
^АВТ: Levy J.; Hancock J.; Ravindran A.; Gross D.; Tamborindeguy C.; Pierson E.
^ЗГЛ: Methods for Rapid and Effective PCR-Based Detection of
'Candidatus Liberibacter solanacearum' From the Insect Vector
Bactericera cockerelli: Streamlining the DNA
Extraction/Purification Process [Оптимизация процессов
экстракции и очистки ДНК при ПЦР-идентификации бактерии
'Candidatus Liberibacter solanacearum' (возбудитель
зебровидной окраски срезов клубней картофеля), выделенной из
переносчика - псиллиды Bactericera cockerelli]
^ВЫХ: Journal of Economic Entomology, 2013; Vol.106,N 3. - P.
1440-1445
^ДАТ: 2013
+Реферат:
^РЕФ: This study provides a protocol for rapid DNA isolation from psyllid vectors (Bactericera cockerelli and Diaphorina citri) that can be used directly with DNA-based methods for the detection of СCandidatus (Ca.) Liberibacter solanacearum,' the bacterial causal agent of potato zebra chip disease and eventually for СCa. Liberibacter asiaticus' the causal agent of huanglongbing disease in citrus. The fast DNA extraction protocol was designed to work with conventional polymerase chain reaction (cPCR) DNA amplification as well as Loop mediated PCR DNA amplification. Direct cPCR of the psyllid 28S rDNA gene from samples prepared using the fast DNA extraction method was as reliable as from samples prepared using standard DNA purification (>97% from live insects) as tested in B. cockerelli. However, samples prepared using the fast DNA extraction method had to be diluted 1:100 in sterile water for reliable amplification, presumably to dilute PCR inhibitors in the crude extract. Similarly, both cPCR and loop mediated PCR DNA amplification detected СCa. Liberibacter' in psyllids infected with either the zebra chip or huanglongbing pathogen equally well from diluted samples prepared using the fast DNA extraction method or from samples prepared using a DNA purification step. In addition to being reliable, the time required to complete the fast DNA extraction for 10 samples was on average ≈5 min and required no special reagents or laboratory equipment. Thus, the fast DNA extraction method shows strong promise as a rapid, reliable, and expedient method when coupled with PCR-based analyses for detection of СCa. Liberibacter' pathogens in psyllids.
aref1
^TRN: 1463637
^ВИД: Статья из книги
^ЯЗК: Английский
+Индексирование:
^РУБ: 68_37_31_49_18
^УДК: 635.21:632.35
^ТЕР: КАРТОФЕЛЬ. SOLANUM TUBEROSUM. БАКТЕРИАЛЬНЫЕ БОЛЕЗНИ
РАСТЕНИЙ [БАКТЕРИОЗЫ РАСТЕНИЙ]. ФИТОПАТОГЕННЫЕ БАКТЕРИИ.
LIBERIBACTER SOLANACEARUM [CANDIDATUS LIBERIBACTER
PSYLLAUROUS; CANDIDATUS LIBERIBACTER SOLANACEARUM;
LIBERIBACTER PSYLLAUROUS]. ДИАГНОСТИКА (Diagnosis).
ИДЕНТИФИКАЦИЯ (Identification). PCR [АМПЛИФИКАЦИЯ IN
VITRO; ПОЛИМЕРАЗНАЯ ЦЕПНАЯ РЕАКЦИЯ; ПЦР; РЕАКЦИЯ ЦЕПНОЙ
ПОЛИМЕРИЗАЦИИ]. ДНК (DNA) [ДЕЗОКСИРИБОНУКЛЕИНОВЫЕ
КИСЛОТЫ]. ЭКСТРАКЦИЯ [ЭКСТРАГИРОВАНИЕ]. ОЧИСТКА
(Cleaning). ПЕРЕНОСЧИКИ (Vectors). TRIOZA COCKERELLI
[BACTERICERA COCKERELLI].
^РТЗ: GRACILICUTES. HEMIPTERA [ПОЛУЖЕСТКОКРЫЛЫЕ]. HOMOPTERA
[РАВНОКРЫЛЫЕ]. LIBERIBACTER [CANDIDATUS LIBERIBACTER;
CANDIDATUS LIBEROBACTER; LIBEROBACTER]. PSYLLOIDEA
[ЛИСТОБЛОШКИ]. RHIZOBIACEAE. SOLANACEAE [ПАСЛЕНОВЫЕ].
SOLANUM. STERNORRHYNCHA [ШЕЕХОБОТНЫЕ]. TRIOZA. TRIOZIDAE.
БАКТЕРИИ (Bacteria). БОЛЕЗНИ РАСТЕНИЙ (Plant diseases).
ДИАГНОСТИЧЕСКИЕ МЕТОДЫ (Diagnostic techniques).
ИНФЕКЦИОННЫЕ БОЛЕЗНИ РАСТЕНИЙ. КЛУБНЕПЛОДНЫЕ КУЛЬТУРЫ
[КОРНЕКЛУБНЕПЛОДНЫЕ КУЛЬТУРЫ]. КРАХМАЛОНОСНЫЕ КУЛЬТУРЫ
(Starch crops). МЕТОДЫ (Methods).
МОЛЕКУЛЯРНО-ГЕНЕТИЧЕСКИЕ МЕТОДЫ. НАСЕКОМЫЕ (Insects)
[INSECTA]. НУКЛЕИНОВЫЕ КИСЛОТЫ (NUCLEIC ACIDS).
НУКЛЕИНОВЫЕ СОЕДИНЕНИЯ (Nucleic compounds). ОРГАНИЗМЫ.
С-Х КУЛЬТУРЫ. ТЕХНИЧЕСКИЕ КУЛЬТУРЫ (Industrial crops).
ТЕХНОЛОГИЧЕСКИЕ ПРОЦЕССЫ (Operational processes).
ФИТОПАТОГЕНЫ. ЧЛЕНИСТОНОГИЕ (Arthropods) [ARTHROPODA].
^КЛС: зебровидная окраска срезов клубней.