^ШХР: *http://jee.oxfordjournals.org/content/by/year
^АВТ: Shihao Tang; Xueru Song; Lin Xue; Xinwei Wang ; Xiufang Wang; Pengjun Xu; Guangwei Ren
^ЗГЛ: Characterization and Distribution Analysis of a Densovirus Infecting Myzus persicae nicotianae (Hemiptera: Aphididae) [Характеристика и анализ частоты встречаемости денсовируса, инфицирующего тлю Myzus persicae nicotianae (Hemiptera: Aphididae). Китай]
^ВЫХ: Journal of Economic Entomology, 2016; Vol.109,N 2. - P. 580-587
^ДАТ: 2016
+Реферат:

^РЕФ: Densoviruses (DVs) are a group of viruses that contain a linear single-stranded DNA genome between 4-6 kb in length. Herein, we report a DV with a 5,480-nt genome, isolated from tobacco aphid (Myzus persicae nicotianae Blackman), named MpnDV. Unlike the genome of M. persicae densovirus (MpDV), which possesses five open reading frames (ORFs), the genome of MpnDV contains four putative ORFs-the nonstructural protein 1 (NS1) and NS2 from MpnDV are 98 - and 52-amino acids longer than those of MpDV, respectively, at the N-terminus, and the capsid proteins (VP) are 102 amino acids longer at the C-terminus than those of MpDV. Mapping of the MpnDV transcripts by RACE method indicated that the ORF of NS2 started at nt 340 and the right two putative ORFs were combined together by deleting two introns, one of 95 bp located at nt 2,932-3,026 and the other of 145 bp located at nt 4,715-4,859, suggesting transcript mapping was necessary for analyzing of genome organization. Alignment analysis indicated that MpnDV shows 97% sequence identity with MpDV, and that the shortened ORFs resulted from nucleotide indels, suggesting MpnDV and MpDV were two isolates of the same virus. Thus, MpnDV and MpDV clustered together in a tree-based analysis. The prevalence of MpnDV infection in wild populations of tobacco aphids differed among 29 locations; 34% of the 622 individuals sampled were positive. The genome organization, transcript strategy, and widespread distribution in wild populations suggest that MpnDV might possess a biological function different from that of MpDV. aref1

^TRN: 1640361
^ВИД: Статья из книги
^ЯЗК: Английский
+Индексирование:
^РУБ: 68_37_29_17_28; 68_37_29_13_17
^УДК: 632.752.2; 632.937.16
^ТЕР: MYZUS PERSICAE [MYZODES PERSICAE; ПЕРСИКОВАЯ ТЛЯ; ТЛЯ ПЕРСИКОВАЯ]. PARVOVIRIDAE. ВИРУСЫ НАСЕКОМЫХ (Insect viruses) [ЭНТОМОПАТОГЕННЫЕ ВИРУСЫ]. ДНК (DNA) [ДЕЗОКСИРИБОНУКЛЕИНОВЫЕ КИСЛОТЫ]. ГЕНОМЫ (Genomes). СЕКВЕНИРОВАНИЕ БЕЛКОВ (protein sequencing) [ПОСЛЕДОВАТЕЛЬНОСТЬ АМИНОКИСЛОТ]. КИТАЙ (China) [КНР].
^РТЗ: APHIDIDAE. APHIDOIDEA [APHIDINEA; APHIDS; ТЛИ]. HEMIPTERA [ПОЛУЖЕСТКОКРЫЛЫЕ]. HOMOPTERA [РАВНОКРЫЛЫЕ]. MYZUS [MYZODES]. STERNORRHYNCHA [ШЕЕХОБОТНЫЕ]. АЗИЯ (Asia). БИОХИМИЧЕСКИЕ МЕТОДЫ. ВИРУСЫ ЖИВОТНЫХ (Animal viruses). ВОСТОЧНАЯ АЗИЯ (East Asia). ЕВРАЗИЯ (Eurasia). МЕТОДЫ (Methods). НАСЕКОМЫЕ (Insects) [INSECTA]. НУКЛЕИНОВЫЕ КИСЛОТЫ (NUCLEIC ACIDS). НУКЛЕИНОВЫЕ СОЕДИНЕНИЯ (Nucleic compounds). СЕКВЕНИРОВАНИЕ (sequencing). СТРАНЫ АТЭС. СТРАНЫ БРИКС. СТРАНЫ МИРА. ЧЛЕНИСТОНОГИЕ (Arthropods) [ARTHROPODA].
^КЛС: Densovirus.

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