^ШХР: *http://jee.oxfordjournals.org/content/by/year
^АВТ: Xing-kui An; Mao-lin Hou; Yu-di Liu
^ЗГЛ: Reference Gene Selection and Evaluation for Gene Expression Studies Using qRT-PCR in the White-Backed Planthopper, Sogatella furcifera (Hemiptera: Delphacidae) [Выбор и анализ стабильности экспрессии референсных генов у дельфациды Sogatella furcifera (Hemiptera: Delphacidae), вредителя риса, методом qRT-PCR. (Китай)]
^ВЫХ: Journal of Economic Entomology, 2016; Vol.109,N 2. - P. 879-886
^ДАТ: 2016
+Реферат:

^РЕФ: The white-backed planthopper, Sogatella furcifera (Hemiptera, Delphacidae), is one of the most devastating rice pests. For a better control strategy, various genetic studies have been conducted using reverse-transcription quantitative real-time polymerase chain reaction (qRT-PCR). The appropriate application of qRT-PCR requires reliable endogenous controls; however, studies on this aspect of the white-backed planthopper are lacking. In the present study, nine commonly used reference genes, elongation factor 1-α (EF1-α), polyubiquitin (UB), ribosomal protein S18 (RPS18), actin 1 (ACT), α-1 tubulin (TUB), glyceraldehyde-3-phosphate (GAPDH), ribosomal protein L9 (RPL9), ribosomal protein L10 (RPL10), and 18S ribosomal RNA (18S), were evaluated by qRT-PCR for their expression stability under four different experimental conditions (different developmental stages, acquisition of Southern rice black-streaked dwarf virus (SRBSDV), different tissues, and different temperature stress). These results were analyzed using four software programs (geNorm, NormFinder, BestKeeper, and the delta Ct method) and a Web-based comprehensive tool RefFinder to compare and rank candidate reference genes. According to the results of RefFinder analysis, which integrates the abovementioned four software programs, TUB was ranked as the most suitable reference gene at different developmental stages and under different temperature stress, and GAPDH and RPL9 showed the highest stability for acquisition of SRBSDV and different tissues, respectively. These results will provide a solid foundation for future gene expression study on the white-backed planthopper, and also will give aids in establishing a standardized qRT-PCR procedure for other related insects. aref1

^TRN: 1641752
^ВИД: Статья из книги
^ЯЗК: Английский
+Индексирование:
^РУБ: 68_37_29_29_17
^УДК: 633.18:632.753.1
^ТЕР: РИС. ORYZA SATIVA. НАСЕКОМЫЕ-ВРЕДИТЕЛИ (Pest insects) [ВРЕДНЫЕ НАСЕКОМЫЕ]. SOGATELLA FURCIFERA [SOGATA FURCIFERA]. RT-PCR [ОБРАТНО-ТРАНСКРИПТАЗНАЯ ПОЛИМЕРАЗНАЯ ЦЕПНАЯ РЕАКЦИЯ; ОТ-ПЦР]. ЭКСПРЕССИЯ ГЕНОВ (Gene expression). КИТАЙ (China) [КНР].
^РТЗ: AUCHENORRHYNCHA [ЦИКАДОВЫЕ]. DELPHACIDAE [ДЕЛЬФАЦИДЫ; СВИНУШКИ]. FULGOROIDEA. HEMIPTERA [ПОЛУЖЕСТКОКРЫЛЫЕ]. HOMOPTERA [РАВНОКРЫЛЫЕ]. ORYZA. PCR [АМПЛИФИКАЦИЯ IN VITRO; ПОЛИМЕРАЗНАЯ ЦЕПНАЯ РЕАКЦИЯ; ПЦР; РЕАКЦИЯ ЦЕПНОЙ ПОЛИМЕРИЗАЦИИ]. POACEAE [GRAMINEAE; ЗЛАКИ; МЯТЛИКОВЫЕ]. SOGATELLA [SOGATODES]. АЗИЯ (Asia). ВОСТОЧНАЯ АЗИЯ (East Asia). ВРЕДИТЕЛИ РАСТЕНИЙ (Pests of plants). ВРЕДНЫЕ БЕСПОЗВОНОЧНЫЕ. ВРЕДНЫЕ ОРГАНИЗМЫ. ВРЕДНЫЕ ЧЛЕНИСТОНОГИЕ. ЕВРАЗИЯ (Eurasia). ЗЕРНОВЫЕ КУЛЬТУРЫ (Grain crops). МЕТОДЫ (Methods). МОЛЕКУЛЯРНО-ГЕНЕТИЧЕСКИЕ МЕТОДЫ. НАСЕКОМЫЕ (Insects) [INSECTA]. С-Х КУЛЬТУРЫ. СТРАНЫ АТЭС. СТРАНЫ БРИКС. СТРАНЫ МИРА. ЧЛЕНИСТОНОГИЕ (Arthropods) [ARTHROPODA].
^КЛС: референсные гены.

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