^ШХР: *www.sciencedirect.com/science/journal/03781135
(www.sciencedirect)
^АВТ: Irrgang A.; Hammerl J.A.; Falgenhauer L.; Guiral E.; Fischer J.; Guerra B.; Chakraborty T.; Kasbohrer A.
^ЗГЛ: Diversity of CTX-M-1-producing E. coli from German food
samples and genetic diversity of the blaCTX-M-1 region on
IncI1 ST3 plasmids [Идентификация и
молекулярно-генетическая характеристика продуцирующих
beta-лактамазу изолятов Escherichia coli из образцов продуктов
питания в ФРГ (изучение генетического разнообразия региона bla
CTX-M-1, ассоциированного с наличием плазмиды IncI1 ST3)]
^ВЫХ: Veterinary Microbiology, 2018; Vol.221,. - P. 98-104
^ДАТ: 2018
+Реферат:
^РЕФ: Highlights Х CTX-M-1 was the most abundant β-lactamase in E. coli from German food samples. Х PFGE and MLST revealed a high diversity of CTX-M-1-producing E. coli. Х dissemination of blaCTX-M-1 was mainly driven by the transmission of an IncI1 ST3 plasmid. Abstract Antimicrobial resistance to cephalosporins is commonly mediated by extended-spectrum β-lactamases (ESBL) or plasmidic AmpC β-lactamases (pAmpC). In livestock blaCTX-M-1 is the most frequently detected ESBL-encoding gene. As transmission to consumers through contaminated food is often proposed, this study characterized ESBL/pAmpC-producing E. coli collected from food samples. Therefore, samples from food products of animal origin and vegetables were screened for phenotypically resistant E. coli by selective cultivation. The ESBL genotype was confirmed for 404 isolates with the majority of them (n = 212) harboring the blaCTX-M-1 gene. PFGE and MLST analyses as well as plasmid characterization were carried out for 89 isolates, selected under epidemiological aspects. In addition, 44 isolates were investigated by whole genome sequencing and/or sequencing of their plasmids on an Illumina Miseq platform. MLST and PFGE indicated a diverse population of CTX-M-1-producing E. coli in German food samples with no spread of single clonal lineages. The majority of the isolates harbored the blaCTX-M-1 gene on IncI1 plasmids. Frequently, the gene was associated with the ISEcp1 element and located on a ∼100 kb IncI1 plasmid depicting the plasmid multilocus sequence type (ST) 3. The blaCTX-M-1 gene and its flanking sequences were located within the shufflon of the type IV pilus region in diverse orientations. In conclusion, dissemination of the CTX-M-1 β-lactamase within food samples of animal origin is driven by the transmission of a ∼100 kb large IncI1 ST3 plasmid. Apart from conjugal transfer of IncI1 ST3 plasmids the transmission of the blaCTX-M-1 gene might be further promoted through mobilization due to its location within a recombination hot-spot of IncI1 plasmids.
aref1
^TRN: 1817352
^ВИД: Статья из книги
^ЯЗК: Английский
+Индексирование:
^РУБ: 65_09_03; 68_41_31_29
^УДК: 664.019; 614.31:664
^ТЕР: ПРОДУКТЫ ПИТАНИЯ (Foods) [ПИЩЕВЫЕ ПРОДУКТЫ;
ПРОДОВОЛЬСТВИЕ]. ВЕТЕРИНАРНО-САНИТАРНАЯ ЭКСПЕРТИЗА.
ESCHERICHIA COLI [КИШЕЧНАЯ ПАЛОЧКА]. ИЗОЛЯТЫ.
ИДЕНТИФИКАЦИЯ (Identification). PCR [АМПЛИФИКАЦИЯ IN
VITRO; ПОЛИМЕРАЗНАЯ ЦЕПНАЯ РЕАКЦИЯ; ПЦР; РЕАКЦИЯ ЦЕПНОЙ
ПОЛИМЕРИЗАЦИИ]. ДНК (DNA) [ДЕЗОКСИРИБОНУКЛЕИНОВЫЕ
КИСЛОТЫ]. СЕКВЕНИРОВАНИЕ (sequencing). ГЕНОМНЫЙ АНАЛИЗ.
ПЛАЗМИДЫ (Plasmids) [ЭПИСОМЫ]. АНТИБИОТИКИ (Antibiotics).
УСТОЙЧИВОСТЬ К ЛЕКАРСТВАМ (Drug resistance). БЕЗОПАСНОСТЬ
ПРОДУКТОВ ПИТАНИЯ (Food safety). ФРГ (FRG) [ГЕРМАНИЯ].
^РТЗ: ENTEROBACTERIACEAE [ЭНТЕРОБАКТЕРИИ]. ESCHERICHIA.
GRACILICUTES. БИОЛОГИЯ (Biology). БИОХИМИЧЕСКИЕ МЕТОДЫ.
ГЕНЕТИКА (Genetics). ГЕНЕТИЧЕСКИЙ АНАЛИЗ. ДИАГНОСТИЧЕСКИЕ
МЕТОДЫ (Diagnostic techniques). ЕВРАЗИЯ (Eurasia). ЕВРОПА
(Europe). ЗАПАДНАЯ ЕВРОПА (Western Europe). КАЧЕСТВО
(Quality). ЛЕКАРСТВЕННЫЕ СРЕДСТВА (Drugs) [ЛЕКАРСТВА;
ЛЕКАРСТВЕННЫЕ ПРЕПАРАТЫ]. МЕТОДЫ (Methods).
МОЛЕКУЛЯРНО-ГЕНЕТИЧЕСКИЕ МЕТОДЫ. НАУКИ (science).
НУКЛЕИНОВЫЕ КИСЛОТЫ (NUCLEIC ACIDS). НУКЛЕИНОВЫЕ
СОЕДИНЕНИЯ (Nucleic compounds). ПРОТИВОМИКРОБНЫЕ СРЕДСТВА
(Antimicrobials) [АНТИБАКТЕРИАЛЬНЫЕ СРЕДСТВА].
ПРОТОПЛАЗМА (Protoplasm). САНИТАРИЯ (Sanitation). СТРАНЫ
ЕС. СТРАНЫ МИРА. СТРУКТУРА КЛЕТКИ (Cell structure)
[КЛЕТКА; КЛЕТКА БИОЛОГИЧЕСКАЯ]. УСТОЙЧИВОСТЬ К ХИМИЧЕСКИМ
ВЕЩЕСТВАМ (Resistance to chemicals). УСТОЙЧИВОСТЬ
[РЕЗИСТЕНТНОСТЬ; СОПРОТИВЛЯЕМОСТЬ; УСТОЙЧИВОСТЬ К
ПОВРЕЖДАЮЩИМ ФАКТОРАМ]. ЦЕНТРАЛЬНАЯ ЕВРОПА. ЦИТОПЛАЗМА
(Cytoplasm). ЦИТОПЛАЗМАТИЧЕСКИЕ ВКЛЮЧЕНИЯ. ЭКСПЕРТИЗА.