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^ШХР: *http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1365-3059 /issues (http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1365-3059/ issues)
^АВТ: Duarte A.; Maleita C.; Egas C.; Abrantes I. ; Curtis R.
^ЗГЛ: Significant effects of RNAi silencing of the venom allergen-like protein (Mhi-vap-1) of the root-knot nematode Meloidogyne hispanica in the early events of infection [Роль опосредованного РНК-интерференцией молчания гена аллерген-подобного белка (Mhi-vap-1) субвентральных эзофагиальных желез инвазионных личинок галловой нематоды Meloidogyne hispanica в обнаружении, заражении растения-хозяина (томат), развитии и размножении паразита. (Португалия. Великобритания)]
^ВЫХ: Plant Pathology, 2017; Vol.66,N 8. - P. 1329-1337
^ДАТ: 2017
^ПРМ: Bibliogr.:p.1336-1337
+Реферат:

^РЕФ: Gene silencing using RNAi is a powerful tool for functional analysis of nematode genes and can provide a new strategy for the management of root-knot nematodes. The transcript of the venom allergen-like protein gene (Mhi-vap-1) of Meloidogyne hispanica is localized in the subventral oesophageal gland cells of second-stage juveniles (J2) and the gene is highly transcribed in this developmental nematode stage. The purpose of this study was to assess whether the silencing of the Mhi-vap-1 gene could affect nematode attraction to roots, penetration, development and reproduction in tomato plants. The optimum soaking time to generate silencing of this gene was determined by quantitative RT-PCR analysis of the relative expression of the Mhi-vap-1 gene in the J2 incubated with the soaking solution for 24, 32 and 48 h. At 48 h, the relative expression of Mhi-vap-1 decreased, which indicates that this period of time is optimum to silence this nematode gene using the RNAi method. Silencing of the Mhi-vap-1 gene interfered with the completion of the nematode life cycle and caused a reduction in nematode attraction to roots, penetration and infection of plants. A small difference in the number of females and galls formed was also observed, which caused a small decrease in the nematode reproduction factor. The use of RNAi silencing of the Meloidogyne effector gene Mhi-vap-1 showed that this gene is important for the plant-nematode interaction during the early events of infection and could be a target gene for anti-nematode strategies. aref1

^TRN: 1821634
^ВИД: Статья из книги
^ЯЗК: Английский
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