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^: *http://www.tandfonline.com/loi/cbst20N.VvoyhdKLTGg (http://www.tandfonline.com/loi/cbst20N.VvoyhdKLTGg)
^: Dedjell A.; Cliquet S.
^: Media and culturing protocol using a full 25 factorial design for the production of submerged aggregates by the potential bio-herbicide Plectosporium alismatis against weed species of Alismataceae [ Plectosporium alismatis ( (Alisma plantago-aquatica)), , - (- ). . ]
^: Biocontrol Science & Technology, 2019; Vol.29,N 4. - P. 308-324
^: 2019
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^: The potential bio-herbicide Plectosporium alismatis produces drying and UV tolerant micro-sclerotia-like structures named aggregates, effective against the weed Alisma plantago-aquatica. In this study, we evaluated (i) optimal liquid culture conditions that supported the high dry weight, conidia and aggregate yields and (ii) stress-tolerance and effectiveness of aggregates. Using a full factorial 25 design, we studied the impact of agitation (A), glucose concentration (B), Tween 80 concentration (C), malt extract concentration (D), and inoculum density (E), on P. alismatis growth yields. The inoculum density (E), the agitation rate (A), and 3-factor interactions involving E and/or A had significant effects on aggregate yields (average 213 aggregates mL−1; main effect of E: +112.87; main effect of A: −74.81), but no significant effect on conidial yields and dry weight. The agitation was maintained at 100 rpm and increasing numbers of conidia were inoculated into the culture media. Maximal aggregate yields (3.6 × 103 aggregates mL−1) were obtained with an initial conidial density of 4 × 106 conidia mL−1. While freshly-harvested, dried and/or UV exposed aggregates germinated at high rates (respectively: 100%; 99%; 76%; 85%), sporogenesis significantly decreased after stress exposure. In bioassays using leaf discs of A. plantago-aquatica, chlorosis and/or necrosis was observed after 8 days incubation, regardless of whether aggregates had been exposed to desiccation and/or UV-radiations prior to application on leaf discs. These data provide a cultural protocol for the production of high numbers of UV and drying resistant aggregates effective against weed species of Alismatacae. aref1

^TRN: 1849904
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