-
()
-


@Mail.ru .
[ ]

^: 3559 2019 4
^: .. ( - - . . . . . . ). - . ; .. ; ..
^: [ ] = Receptor profile of blood lymphocytes of vaccinated sheep
^: , 2019; N 4. - . 8-10
^: 2019
^: . ..-.:.10
+

^: , , , . , CD- . CD- . IgM, CD19,CD5,CD4,CD8, CD16.

aref2 2 There is a constant interaction of ligands with receptors of immunocompetent cells, which leads to the redistribution of the latter on the surface of lymphocytes and a change in the functional activity of the cell and, consequently, its phenotype. In animals, these processes are not well understood, since the identification of lymphocytes is a difficult task due to the lack of antibodies against animal CD receptors in the domestic market. In the present work, experimental data on the dynamics of expression of CD markers by lymphocytes of sheep blood are given. For this, the immunoperoxidase staining method with monoclonal antibodies to IgM, CD19, CD5, CD4, CD8, CD16 was used. For the experiment, blood samples were taken from 11 sheep of the Romanov breed aged 5-6 years. Animals (n = 5) were immunized subcutaneously with inactivated colibacillosis vaccine. Studies were performed before and after vaccination on the 1st and 2nd day of the immune response. As studies have shown, the parameters of B cells obtained by the IPO method using monoclonal antibodies to bovine IgM and to the human B-lymphocyte CD19 receptor have a high degree of correlation (r = 0.95). On this basis, it can be concluded about partial interspecific homology of B-lymphocyte receptors. As a result of the research, it was established that on the 1st day of the post-vaccination immune response in the peripheral blood of sheep, the population of killer cells, granulocytes, macrophages and the cytotoxic lymphocytes increased. As a result of immunophenotyping by IPO using monoclonal antibodies to markers of bovine and human lymphocytes, it was established that the phenotypic characteristic of the blood lymphocytes of the first day post-vaccine immune response to an inactivated vaccine was sIgM-/CD19-/CD5-/CD4-/ CD8+/ CD16+, respectively , second day - sIgM+/CD19+/CD5-/CD4-/ CD8-/ CD16-.

^TRN: 1862288
^:
^:
+



        

1998-2021 ©