^АВТ: Yu-Shin Nai; Chou-Ming Lo
^ЗГЛ: Persistent PnV (Perina nuda virus) infection in a
heterologous Lymantria xylina cell line, NTU-LY
[Персистентная инфекция гетерологичной клеточной линии
Lymantria xylina вирусом PnV (Perina nuda virus). Китай]
^ВЫХ: Biocontrol Science & Technology, 2020; Vol.30,N 9. - P.
^РЕФ: The NTU-LY-1 cell line is a Lymantria xylina cell line; however, during cell passage, obvious cytopathic effects (CPEs) were observed under light microscopy. Round inclusion body (IB)-like particles and a few irregular larger IB-like particles were found in the cytoplasm of NTU-LY-1 cells. To further investigate the CPEs in NTU-LY-1 cells, reverse transcription - PCR screening of cytoplasmic polyhedrosis virus, infectious flacherie virus and Perina nuda virus (PnV) was performed. The results indicated that NTU-LY-1 cells were persistently infected with PnV from days 1 to 15 of culture. NTU-LY-1 cells were further subjected to Western blotting and indirect immunofluorescence assays to confirm PnV infection. Based on transmission electronic microscopy observations, the CPEs in NTU-LY-1 cells were different from those in the PnV-homologous NTU-Pn-HF cell line (a cell line of P. nuda). Membrane-bound IBs were found in newly seeded NTU-LY-1 cells. Lysed NTU-LY-1 cells formed many microsomes after 15 days of culture, and three different types of IBs could be distinguished among the microsomes, including membrane-bound, fibril-bound and sheath-bound IBs. After 15 days of culture, the cytoplasm of un-lysed NTU-LY-1 cells contained zipper-like arrays of non-membrane-bound virogenic stroma and parallel rows of viral particles that were restricted by two membranes. These viral particles were purified and showed a PnV morphology. This is the first study to show persistent PnV infection in a heterologous cell line. The findings suggest a new strategy for microbial control; moreover, the findings could support the development of a model for membrane-associated replication of positive-sense single-stranded RNA viruses.
^ВИД: Статья из книги
^РУБ: 68_37_29_17_28; 68_37_29_13_17
^УДК: 632.787; 632.937.16
^ТЕР: LYMANTRIA XYLINA [LYMANTRIA NIGRICOSTA]. КУЛЬТУРА ТКАНЕЙ
(Tissue culture). ВИРУСЫ НАСЕКОМЫХ (Insect viruses)
[ЭНТОМОПАТОГЕННЫЕ ВИРУСЫ]. КИТАЙ (China) [КНР].
^РТЗ: LEPIDOPTERA [ЧЕШУЕКРЫЛЫЕ]. LYMANTRIA [PORTHETRIA].
LYMANTRIIDAE [ВОЛНЯНКИ]. АЗИЯ (Asia). ВИРУСЫ ЖИВОТНЫХ
(Animal viruses). ВОСТОЧНАЯ АЗИЯ (East Asia). ЕВРАЗИЯ
(Eurasia). КУЛЬТУРА IN VITRO (In vitro culture).
НАСЕКОМЫЕ (Insects) [INSECTA]. СТРАНЫ АТЭС. СТРАНЫ БРИКС.
СТРАНЫ МИРА. ЧЛЕНИСТОНОГИЕ (Arthropods) [ARTHROPODA].
^КЛС: Perina nuda virus. Iflavirus.